Abstract: A Bacillus stearothermophilus strain shows the special property of decomposing sucrose to acid in a shorter time than other sugars and moreover at a high speed independent of the sucrose concentration. This property is pronounced so as to allow quantitative sucrose determination to be built up on it. The method is not interferred by, for instance, a fiftyfold excess of invert sugar. The chief apparatus needed are a temperable incubation vessel and a recording pH-meter. The analysis proper includes the dosage into an incubation vessel of samples, calibration solutions, and lye, and that without the necessity of sterilising the material, and the reading of the data from the pH-diagram at intervals ranging from about 15 to 30 minutes.
|