|
Abstract: A microbiological technique for quantitative determination of sucrose is described which is based on continuous flow analysis. It can be carried out with normal commercial equipment. Diluted samples are mixed with a continuously cultivated pre-culture of sucrose-selective micro-organisms. After incubation in a tubular fermenter they are subjected to continuous pH measurement. In contrast to methods described in previous papers, the advantages lie in the greater analysis frequency (40 per hour) and continuous operation which is independent of test material. The repetability coefficient of variation, obtainable under ideal conditions (matrix-free solution, maximum response), is 0.3-0.4. Systematic errors caused by the sample matrix can be eliminated or reduced by use of an internal standard or additional use of saccharase. Given examples of application include analytical instructions and reference points for determination of method precision and accuracy in individual cases. Within the beet sugar industry the method is suitable for beet, pulp products, juices and syrup, while outside it can be applied to cane molasses, confectionery and honey. The possibility of application in the field of medicine is indicated. |