| Abstract: This paper describes the measurement of the main monosaccharide component of beet, i.e. glucose, routinely in an ordinary beet laboratory. It uses an optimized enzymatic assay of glucose by the GOD/POD method which can be atomated in various ways because there is no necessity for a dialysis step. This is achieved by the addition of a chelating agent, which prevents the flocculation of aluminium- or lead compounds. Due to the uncomplicated colourimetric measurement, which is simple to perform as the "blue number" method, this method is suitable for serial determinations, as is usual in beet analyses. The glucose contents, determined in samples after aluminium defecation, compared well with the Hexokinase method (HK) results, obtained from beet filtrates, which were clarified according to Carrez. If lead acetate was employed for defecation, glucose losses, ranging from 5 to 10 % (rel.) have to be taken into account. The addition of a chelating agent permits, at least in the case of aluminium defecation, also the use of the HK-method, however at higher cost.
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